1P158 Folding mechanism of actin analyzed by fluorescence microscopy

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Double immunolabeling of neuropeptides in the human hypothalamus as analyzed by confocal laser scanning fluorescence microscopy.

The main goal of this study was to develop a better light microscopic procedure for quantitative study of the cellular co-localization of neuropeptides in adult human brain tissue. To reach this goal, we opted for a method (proved to be optimal on rat brain) in which sections were double immunolabeled with two different fluorophore-conjugated secondary antibodies and analyzed with a confocal la...

متن کامل

Interpreting photoactivated fluorescence microscopy measurements of steady-state actin dynamics.

A continuum model describing the steady-state actin dynamics of the cytoskeleton of living cells has been developed to aid in the interpretation of photoactivated fluorescence experiments. In a simplified cell geometry, the model assumes uniform concentrations of cytosolic and cytoskeletal actin throughout the cell and no net growth of either pool. The spatiotemporal evolution of the fluorescen...

متن کامل

Determine folding mechanism of Lali structure, northern Dezful, Zagros, Iran

     Lali sub-surface structure, with a NW-SE Zagros trending is located in Dezful Embayment. To determine the folding mechanism, structural geometric parameters including limbs dip, amplitude, wavelength, and crestal length were determined in four stages during deformation. In order to investigate the lateral folding mechanism, these geometric parameters were analyzed in three parts in the Lal...

متن کامل

Folding Dynamics of Single GCN-4 Peptides by Fluorescence Resonant Energy Transfer Confocal Microscopy

We have prepared a bichromophoric crosslinked variant of GCN4-P1 for single molecule fluorescence energy transfer experiments (GCN4-Pf). The folding and unfolding fluctuations of single GCN4-Pf molecules are measured in a two channel confocal microscope with which donor and acceptor fluorescence trajectories are measured simultaneously. The energy transfer efficiency is thereby determined and i...

متن کامل

Study of the State of F-Actin in Denervated Muscle Fibre by Polarised UV Fluorescence Microscopy

In order to establish the effect of denervation on the structure of F-actin, polarisation of fluorescence of tryptophan residues of F-actin was determined in intact and denervated ghost muscle fibres (freed from myosin, troponin and tropomyosin) under various conditions. P\\, the polarisation parameter obtained by excitation of the fluorescence of tryptophan residues of F-actin by light polaris...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Seibutsu Butsuri

سال: 2004

ISSN: 0582-4052,1347-4219

DOI: 10.2142/biophys.44.s69_2